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mouse cy5 jackson immuno  (Jackson Immuno)


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    Structured Review

    Jackson Immuno mouse cy5 jackson immuno
    Mouse Cy5 Jackson Immuno, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 701 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy5+donkey+anti+mouse/Cy+5+AffiniPure+Donkey+Anti-Mouse+IgG/pm41922759-764-139-141
    Average 96 stars, based on 701 article reviews
    mouse cy5 jackson immuno - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: 3D in vitro Modelling of Post-Partum Cardiovascular Health reveals Unique Characteristics and Signatures following Hypertensive Disorders in Pregnancy
    Article Snippet: CS were then probed with Galectin-3 (1:100, Abcam, Cambridge, UK), FKBPL (1:100, Proteintech, Rosemont, USA), CD31 (1:10, BD Biosciences, Franklin Lakes, USA), VE-cadherin (1:100, Abcam, Cambridge, UK), αSMA (1:100, Abcam, Cambridge, UK) primary antibodies and incubated at 4°C overnight. .. Following washing, goat antirabbit IgG H&L (Alexa Fluor® 488, Abcam, Cambridge, UK) and goat anti-mouse IgG H&L (Alexa Fluor® 594, Abcam, Cambridge, UK), and Cy5-Donkey anti-mouse (1:142, Jackson ImmunoResearch, Pennsylvania, USA) secondary antibodies were added and incubated at 4°C overnight. .. CSs were then incubated with Vimentin (1:250, Abcam, Cambridge, UK) and Troponin C (1:10, Santa Cruz, California, USA) primary-conjugated antibodies and DAPI (10 μg/mL, Invitrogen, Massachusetts, USA) at 4°C overnight.

    Article Title: Suppressing PDGFRβ Signaling Enhances Myocyte Fusion to Promote Skeletal Muscle Regeneration
    Article Snippet: .. Cells were then incubated at 4°C overnight with primary antibody at 1:100 in blocking buffer The following day cells were washed 3 times for 5 minutes with 1X TBS then incubated with Cy5 Donkey Anti-Mouse (Jackson ImmunoResearch 715-175-150) at 1:100 in 1X TBS for 2 hours in the dark at room temperature. ..

    Article Title: Functional contribution of astrocytic Kir4.1 channels to spasticity after spinal cord injury
    Article Snippet: The primary antibody anti-STAT3 (1/300; 8768, Cell Signaling Technology, MA) was incubated with Cell signal blocking solution (8112, Cell Signaling Technology, MA) for 48 h at 4°C. .. Slides were then washed 3×5 min in PBS and incubated for 2 h with the appropriate secondary antibodies diluted in the blocking solution: Alexa Fluor® Plus 555-donkey anti-rabbit (1/400; A32794, Invitrogen, MA), Cy5-donkey anti-mouse (1/400; 715-175-151, Jackson ImmunoResearch, PA) and/or Alexa Fluor® Plus 555-donkey anti-goat (1/400; A32816, Invitrogen, MA). .. After 3 washes of 5 min in PBS, slides were incubated with Hoechst 33342 (2 μM in PBS; 62249, Thermo Fisher Scientific, MA) for 5 min and washed again 3x5 min before being mounted with a homemade gelatinous aqueous medium.

    Article Title: 3D in vitro modelling of post-partum cardiovascular health reveals unique characteristics and signatures following hypertensive disorders in pregnancy
    Article Snippet: CSs were then probed with Galectin-3 (1:100, Abcam, Cambridge, UK), FKBPL (1:100, Proteintech, Rosemont, USA), CD31 (1:10, BD Biosciences, Franklin Lakes, USA), VE-cadherin (1:100, Abcam, Cambridge, UK), αSMA (1:100, Abcam, Cambridge, UK) primary antibodies and incubated at 4 °C overnight. .. Following washing, goat anti-rabbit IgG H&L (Alexa Fluor ® 488, Abcam, Cambridge, UK) and goat anti-mouse IgG H&L (Alexa Fluor ® 594, Abcam, Cambridge, UK), and Cy5-Donkey anti-mouse (1:142, Jackson ImmunoResearch, Pennsylvania, USA) secondary antibodies were added and incubated at 4 °C overnight. .. CSs were then incubated with Vimentin (1:250, Abcam, Cambridge, UK) and Troponin C (1:10, Santa Cruz, California, USA) primary-conjugated antibodies and DAPI (10 μg/ml, Invitrogen, Massachusetts, USA) at 4 °C overnight.

    Blocking Assay:

    Article Title: Suppressing PDGFRβ Signaling Enhances Myocyte Fusion to Promote Skeletal Muscle Regeneration
    Article Snippet: .. Cells were then incubated at 4°C overnight with primary antibody at 1:100 in blocking buffer The following day cells were washed 3 times for 5 minutes with 1X TBS then incubated with Cy5 Donkey Anti-Mouse (Jackson ImmunoResearch 715-175-150) at 1:100 in 1X TBS for 2 hours in the dark at room temperature. ..

    Article Title: Functional contribution of astrocytic Kir4.1 channels to spasticity after spinal cord injury
    Article Snippet: The primary antibody anti-STAT3 (1/300; 8768, Cell Signaling Technology, MA) was incubated with Cell signal blocking solution (8112, Cell Signaling Technology, MA) for 48 h at 4°C. .. Slides were then washed 3×5 min in PBS and incubated for 2 h with the appropriate secondary antibodies diluted in the blocking solution: Alexa Fluor® Plus 555-donkey anti-rabbit (1/400; A32794, Invitrogen, MA), Cy5-donkey anti-mouse (1/400; 715-175-151, Jackson ImmunoResearch, PA) and/or Alexa Fluor® Plus 555-donkey anti-goat (1/400; A32816, Invitrogen, MA). .. After 3 washes of 5 min in PBS, slides were incubated with Hoechst 33342 (2 μM in PBS; 62249, Thermo Fisher Scientific, MA) for 5 min and washed again 3x5 min before being mounted with a homemade gelatinous aqueous medium.

    Staining:

    Article Title: Cxcr4 regulates a pool of adipocyte progenitors and contributes to adiposity in a sex-dependent manner
    Article Snippet: .. Alternatively, cells were fixed, permeabilized, and stained for BrdU (eFluor 450; 1:200; Invitrogen 48-5071-41), CD68 (AlexaFluor 647; 1:200; Biolegend 137003), Cxcr4/CD184 (APC; 1:400; Biolegend 146507), CD45 (PE-Cy7; 1:200; Invitrogen 25-0451-82), DPP-4/CD26 (PE; 1:100; Biolegend 137803), Pdgfrα/ CD140a (1:200; Invitrogen 14-1401-82), Estrogen Receptor Alpha (1:200; abcam 32063), or Phosphorylated P38/MAPK (1:200 cell signaling 9211) and conjugated to Cy5 donkey anti-rabbit: (Invitrogen A10523, lot 2156245) or Cy5 donkey anti-mouse (Jackson Immunoresearch 715-175-150) fluorphore , . ..



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